Thin-layer chromatography of pigments from reaction center particles of Rhodopseudomonas spheroides
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چکیده
منابع مشابه
Thin-layer chromatography of pigments from reaction center particles of Rhodopseudomonas spheroides.
During the past few years a number of papers have been published, dealing with the isolation of photochemically active reaction center particles from photosynthetic bacteria (e.g. refs 1-6). In these particles the light-harvesting bacteriochlorophyll and most of the other accessory pigments such as carotenoids, have been removed. The particles still contain specialized bactefiochlorophylls, P8o...
متن کاملCharacterization of the pigments in reaction center preparations from Rhodopseudomonas spheroides.
The pigments in reaction center preparations from Rhodopseudomonas spheroides strain R-26 have been characterized. The absorption spectra and magnesium content of both intact preparations and organic extracts as well as thin layer chromatography of the extracts indicate that the pigment complex of the reaction center contains both bacteriopheophytin a and bacteriochlorophyll a. The functional u...
متن کاملSpectral properties of reaction center preparations from Rhodopseudomonas spheroides.
The absorption, circular dichroism, and fluorescence spectra have been measured on oxidized and reduced reaction center preparations from Rhodopseudomonas spheroides strain R-26 at 295 and 77” K. The spectra indicate that the 4 bacteriochlorophyll a and 2 bacteriopheophytin a molecules in the reaction center are contained in a single pigment complex. The absorption bands at 893, 804, and ‘760 i...
متن کاملA partial reaction of delta-aminolaevulinate synthetase from Rhodopseudomonas spheroides.
microsomal fraction. The properties of the microsomal phosphorylcholinetransferase enzyme are similar to those reported for that from spinach leaves (Devor & Mudd, 1971), except that exogenous diglyceride was necessary for maximal rates, presumably because the activity of endogenous lipases was not high enough to generate a sufficient amount. These results indicate that, whereas the CDP-base pa...
متن کاملElectron microscopy of chromatophores of Rhodopseudomonas spheroides.
Gibson, K. D. (St. Mary's Hospital Medical School, London, England). Electron microscopy of Rhodopseudomonas spheroides. J. Bacteriol. 90:1059-1072. 1965.-Fixed and stained chromatophores and whole cells of anaerobically grown Rhodopseudomonas spheroides were examined in thin sections in the electron microscope. Both purified chromatophores and intracellular membrane-bound vesicles had exactly ...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Bioenergetics
سال: 1972
ISSN: 0005-2728
DOI: 10.1016/0005-2728(72)90248-4